Cargando…

The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country

BACKGROUND: The vitamin D receptor (VDR) gene is expressed abundantly in different tissues; including adipocytes and pancreatic beta cells. The rs1544410 or BsmI single nucleotide polymorphism (SNP) in the intronic region of the VDR gene has been previously associated with vitamin D levels, obesity...

Descripción completa

Detalles Bibliográficos
Autores principales: Rahmadhani, Rayinda, Zaharan, Nur Lisa, Mohamed, Zahurin, Moy, Foong Ming, Jalaludin, Muhammad Yazid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5472260/
https://www.ncbi.nlm.nih.gov/pubmed/28617856
http://dx.doi.org/10.1371/journal.pone.0178695
_version_ 1783244091233927168
author Rahmadhani, Rayinda
Zaharan, Nur Lisa
Mohamed, Zahurin
Moy, Foong Ming
Jalaludin, Muhammad Yazid
author_facet Rahmadhani, Rayinda
Zaharan, Nur Lisa
Mohamed, Zahurin
Moy, Foong Ming
Jalaludin, Muhammad Yazid
author_sort Rahmadhani, Rayinda
collection PubMed
description BACKGROUND: The vitamin D receptor (VDR) gene is expressed abundantly in different tissues; including adipocytes and pancreatic beta cells. The rs1544410 or BsmI single nucleotide polymorphism (SNP) in the intronic region of the VDR gene has been previously associated with vitamin D levels, obesity and insulin resistance. AIMS: This study was aimed to examine the association between BsmI polymorphism and risk of vitamin D deficiency, obesity and insulin resistance in adolescents living in a tropical country. METHODS: Thirteen-year-old adolescents were recruited via multistage sampling from twenty-three randomly selected schools across the city of Kuala Lumpur, Malaysia (n = 941). Anthropometric measurements were obtained. Obesity was defined as body mass index higher than the 95(th) percentile of the WHO chart. Levels of fasting serum vitamin D (25-hydroxyvitamin D (25(OH)D)), glucose and insulin were measured. HOMA-IR was calculated as an indicator for insulin resistance. Genotyping was performed using the Sequenom MassARRAY platform (n = 807). The associations between BsmI and vitamin D, anthropometric parameters and HOMA-IR were examined using analysis of covariance and logistic regression. RESULT: Those with AA genotype of BsmI had significantly lower levels of 25(OH)D (p = 0.001) compared to other genotypes. No significant differences was found across genotypes for obesity parameters. The AA genotype was associated with higher risk of vitamin D deficiency (p = 0.03) and insulin resistance (p = 0.03) compared to GG. The A allele was significantly associated with increased risk of vitamin D deficiency compared to G allele (adjusted odds ratio (OR) = 1.63 (95% Confidence Interval (CI) 1.03–2.59, p = 0.04). In those with concurrent vitamin D deficiency, having an A allele significantly increased their risk of having insulin resistance compared to G allele (adjusted OR = 2.66 (95% CI 1.36–5.19, p = 0.004). CONCLUSION: VDR BsmI polymorphism was significantly associated with vitamin D deficiency and insulin resistance, but not with obesity in this population.
format Online
Article
Text
id pubmed-5472260
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-54722602017-07-03 The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country Rahmadhani, Rayinda Zaharan, Nur Lisa Mohamed, Zahurin Moy, Foong Ming Jalaludin, Muhammad Yazid PLoS One Research Article BACKGROUND: The vitamin D receptor (VDR) gene is expressed abundantly in different tissues; including adipocytes and pancreatic beta cells. The rs1544410 or BsmI single nucleotide polymorphism (SNP) in the intronic region of the VDR gene has been previously associated with vitamin D levels, obesity and insulin resistance. AIMS: This study was aimed to examine the association between BsmI polymorphism and risk of vitamin D deficiency, obesity and insulin resistance in adolescents living in a tropical country. METHODS: Thirteen-year-old adolescents were recruited via multistage sampling from twenty-three randomly selected schools across the city of Kuala Lumpur, Malaysia (n = 941). Anthropometric measurements were obtained. Obesity was defined as body mass index higher than the 95(th) percentile of the WHO chart. Levels of fasting serum vitamin D (25-hydroxyvitamin D (25(OH)D)), glucose and insulin were measured. HOMA-IR was calculated as an indicator for insulin resistance. Genotyping was performed using the Sequenom MassARRAY platform (n = 807). The associations between BsmI and vitamin D, anthropometric parameters and HOMA-IR were examined using analysis of covariance and logistic regression. RESULT: Those with AA genotype of BsmI had significantly lower levels of 25(OH)D (p = 0.001) compared to other genotypes. No significant differences was found across genotypes for obesity parameters. The AA genotype was associated with higher risk of vitamin D deficiency (p = 0.03) and insulin resistance (p = 0.03) compared to GG. The A allele was significantly associated with increased risk of vitamin D deficiency compared to G allele (adjusted odds ratio (OR) = 1.63 (95% Confidence Interval (CI) 1.03–2.59, p = 0.04). In those with concurrent vitamin D deficiency, having an A allele significantly increased their risk of having insulin resistance compared to G allele (adjusted OR = 2.66 (95% CI 1.36–5.19, p = 0.004). CONCLUSION: VDR BsmI polymorphism was significantly associated with vitamin D deficiency and insulin resistance, but not with obesity in this population. Public Library of Science 2017-06-15 /pmc/articles/PMC5472260/ /pubmed/28617856 http://dx.doi.org/10.1371/journal.pone.0178695 Text en © 2017 Rahmadhani et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rahmadhani, Rayinda
Zaharan, Nur Lisa
Mohamed, Zahurin
Moy, Foong Ming
Jalaludin, Muhammad Yazid
The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country
title The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country
title_full The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country
title_fullStr The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country
title_full_unstemmed The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country
title_short The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country
title_sort associations between vdr bsmi polymorphisms and risk of vitamin d deficiency, obesity and insulin resistance in adolescents residing in a tropical country
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5472260/
https://www.ncbi.nlm.nih.gov/pubmed/28617856
http://dx.doi.org/10.1371/journal.pone.0178695
work_keys_str_mv AT rahmadhanirayinda theassociationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry
AT zaharannurlisa theassociationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry
AT mohamedzahurin theassociationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry
AT moyfoongming theassociationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry
AT jalaludinmuhammadyazid theassociationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry
AT rahmadhanirayinda associationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry
AT zaharannurlisa associationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry
AT mohamedzahurin associationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry
AT moyfoongming associationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry
AT jalaludinmuhammadyazid associationsbetweenvdrbsmipolymorphismsandriskofvitaminddeficiencyobesityandinsulinresistanceinadolescentsresidinginatropicalcountry