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Autophagy controls centrosome number by degrading Cep63
Centrosome number is associated with the chromosome segregation and genomic stability. The ubiquitin–proteasome system is considered to be the main regulator of centrosome number. However, here we show that autophagy also regulates the number of centrosomes. Autophagy-deficient cells carry extra cen...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473638/ https://www.ncbi.nlm.nih.gov/pubmed/27869116 http://dx.doi.org/10.1038/ncomms13508 |
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author | Watanabe, Yuichiro Honda, Shinya Konishi, Akimitsu Arakawa, Satoko Murohashi, Michiko Yamaguchi, Hirofumi Torii, Satoru Tanabe, Minoru Tanaka, Shinji Warabi, Eiji Shimizu, Shigeomi |
author_facet | Watanabe, Yuichiro Honda, Shinya Konishi, Akimitsu Arakawa, Satoko Murohashi, Michiko Yamaguchi, Hirofumi Torii, Satoru Tanabe, Minoru Tanaka, Shinji Warabi, Eiji Shimizu, Shigeomi |
author_sort | Watanabe, Yuichiro |
collection | PubMed |
description | Centrosome number is associated with the chromosome segregation and genomic stability. The ubiquitin–proteasome system is considered to be the main regulator of centrosome number. However, here we show that autophagy also regulates the number of centrosomes. Autophagy-deficient cells carry extra centrosomes. The autophagic regulation of centrosome number is dependent on a centrosomal protein of 63 (Cep63) given that cells lacking autophagy contain multiple Cep63 dots that are engulfed and digested by autophagy in wild-type cells, and that the upregulation of Cep63 increases centrosome number. Cep63 is recruited to autophagosomes via interaction with p62, a molecule crucial for selective autophagy. In vivo, hematopoietic cells from autophagy-deficient and p62(−/−) mice also contained multiple centrosomes. These results indicate that autophagy controls centrosome number by degrading Cep63. |
format | Online Article Text |
id | pubmed-5473638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-54736382017-06-28 Autophagy controls centrosome number by degrading Cep63 Watanabe, Yuichiro Honda, Shinya Konishi, Akimitsu Arakawa, Satoko Murohashi, Michiko Yamaguchi, Hirofumi Torii, Satoru Tanabe, Minoru Tanaka, Shinji Warabi, Eiji Shimizu, Shigeomi Nat Commun Article Centrosome number is associated with the chromosome segregation and genomic stability. The ubiquitin–proteasome system is considered to be the main regulator of centrosome number. However, here we show that autophagy also regulates the number of centrosomes. Autophagy-deficient cells carry extra centrosomes. The autophagic regulation of centrosome number is dependent on a centrosomal protein of 63 (Cep63) given that cells lacking autophagy contain multiple Cep63 dots that are engulfed and digested by autophagy in wild-type cells, and that the upregulation of Cep63 increases centrosome number. Cep63 is recruited to autophagosomes via interaction with p62, a molecule crucial for selective autophagy. In vivo, hematopoietic cells from autophagy-deficient and p62(−/−) mice also contained multiple centrosomes. These results indicate that autophagy controls centrosome number by degrading Cep63. Nature Publishing Group 2016-11-21 /pmc/articles/PMC5473638/ /pubmed/27869116 http://dx.doi.org/10.1038/ncomms13508 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Watanabe, Yuichiro Honda, Shinya Konishi, Akimitsu Arakawa, Satoko Murohashi, Michiko Yamaguchi, Hirofumi Torii, Satoru Tanabe, Minoru Tanaka, Shinji Warabi, Eiji Shimizu, Shigeomi Autophagy controls centrosome number by degrading Cep63 |
title | Autophagy controls centrosome number by degrading Cep63 |
title_full | Autophagy controls centrosome number by degrading Cep63 |
title_fullStr | Autophagy controls centrosome number by degrading Cep63 |
title_full_unstemmed | Autophagy controls centrosome number by degrading Cep63 |
title_short | Autophagy controls centrosome number by degrading Cep63 |
title_sort | autophagy controls centrosome number by degrading cep63 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473638/ https://www.ncbi.nlm.nih.gov/pubmed/27869116 http://dx.doi.org/10.1038/ncomms13508 |
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