An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species

Vector-free allele exchange (VFAE) is a newly developed protocol for genome editing in Pseudomonas species. Although several parameters have been determined to optimize the procedures for obtaining a stable and high-frequency mutation, numerous false-positive clones still appear on the plate, which...

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Detalles Bibliográficos
Autores principales: Gomaa, Ahmed E., Zhang, Chen, Yang, Zhimin, Shang, Liguo, Jiang, Shijie, Deng, Zhiping, Zhan, Yuhua, Lu, Wei, Lin, Min, Yan, Yongliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474227/
https://www.ncbi.nlm.nih.gov/pubmed/28629206
http://dx.doi.org/10.1186/s13568-017-0425-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5474227/
https://www.ncbi.nlm.nih.gov/pubmed/28629206
http://dx.doi.org/10.1186/s13568-017-0425-y

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