Variables influencing fluorimetric N-sulfoglucosamine sulfohydrolase (SGSH) activity measurement in brain homogenates

Deficient N-sulfoglucosamine sulfohydrolase (SGSH) enzyme activity causes mucopolysaccharidosis (MPS) type IIIA. A fluorimetric SGSH activity assay is commonly used to examine patient cells. Here, we modified this method for brain homogenates and define the parameters for assay linearity. SGSH activ...

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Detalles Bibliográficos
Autores principales: Whyte, Lauren S., Hopwood, John J., Hemsley, Kim M., Lau, Adeline A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5475436/
https://www.ncbi.nlm.nih.gov/pubmed/28652977
http://dx.doi.org/10.1016/j.ymgmr.2015.10.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5475436/
https://www.ncbi.nlm.nih.gov/pubmed/28652977
http://dx.doi.org/10.1016/j.ymgmr.2015.10.005

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