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Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli
Codon usage is one of the factors influencing recombinant protein expression. We were interested in the codon usage of an antibody Fab fragment gene exhibiting extreme toxicity in the E. coli host. The toxic synthetic human Fab gene contained domains optimized by the “one amino acid-one codon” metho...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476587/ https://www.ncbi.nlm.nih.gov/pubmed/28630449 http://dx.doi.org/10.1038/s41598-017-03957-6 |