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Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli

Codon usage is one of the factors influencing recombinant protein expression. We were interested in the codon usage of an antibody Fab fragment gene exhibiting extreme toxicity in the E. coli host. The toxic synthetic human Fab gene contained domains optimized by the “one amino acid-one codon” metho...

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Detalles Bibliográficos
Autores principales:	Kulmala, Antti, Huovinen, Tuomas, Lamminmäki, Urpo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476587/ https://www.ncbi.nlm.nih.gov/pubmed/28630449 http://dx.doi.org/10.1038/s41598-017-03957-6

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