Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves

The increasing urgency for replacement of pathological heart valves is a major stimulus for research on alternatives to glutaraldehyde-treated grafts. New xenogeneic acellular heart valve substitutes that can be repopulated by host cells are currently under investigation. Anionic surfactants, includ...

Descripción completa

Detalles Bibliográficos
Autores principales: Dettin, Monica, Zamuner, Annj, Naso, Filippo, Monteleone, Antonella, Spina, Michele, Gerosa, Gino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476881/
https://www.ncbi.nlm.nih.gov/pubmed/28676861
http://dx.doi.org/10.1155/2017/9274135
_version_ 1783244685023641600
author Dettin, Monica
Zamuner, Annj
Naso, Filippo
Monteleone, Antonella
Spina, Michele
Gerosa, Gino
author_facet Dettin, Monica
Zamuner, Annj
Naso, Filippo
Monteleone, Antonella
Spina, Michele
Gerosa, Gino
author_sort Dettin, Monica
collection PubMed
description The increasing urgency for replacement of pathological heart valves is a major stimulus for research on alternatives to glutaraldehyde-treated grafts. New xenogeneic acellular heart valve substitutes that can be repopulated by host cells are currently under investigation. Anionic surfactants, including bile acids, have been widely used to eliminate the resident cell components chiefly responsible for the immunogenicity of the tissue, even if detergent toxicity might present limitations to the survival and/or functional expression of the repopulating cells. To date, the determination of residual detergent has been carried out almost exclusively on the washings following cell removal procedures. Here, a novel HPLC-based procedure is proposed for the direct quantification of detergent (cholate, deoxycholate, and taurodeoxycholate) residues entrapped in the scaffold of decellularized porcine aortic and pulmonary valves. The method was demonstrated to be sensitive, reproducible, and extendable to different types of detergent. This assessment also revealed that cell-depleted heart valve scaffolds prepared according to procedures currently considered for clinical use might contain significant amount of surfactant.
format Online
Article
Text
id pubmed-5476881
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-54768812017-07-04 Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves Dettin, Monica Zamuner, Annj Naso, Filippo Monteleone, Antonella Spina, Michele Gerosa, Gino Biomed Res Int Research Article The increasing urgency for replacement of pathological heart valves is a major stimulus for research on alternatives to glutaraldehyde-treated grafts. New xenogeneic acellular heart valve substitutes that can be repopulated by host cells are currently under investigation. Anionic surfactants, including bile acids, have been widely used to eliminate the resident cell components chiefly responsible for the immunogenicity of the tissue, even if detergent toxicity might present limitations to the survival and/or functional expression of the repopulating cells. To date, the determination of residual detergent has been carried out almost exclusively on the washings following cell removal procedures. Here, a novel HPLC-based procedure is proposed for the direct quantification of detergent (cholate, deoxycholate, and taurodeoxycholate) residues entrapped in the scaffold of decellularized porcine aortic and pulmonary valves. The method was demonstrated to be sensitive, reproducible, and extendable to different types of detergent. This assessment also revealed that cell-depleted heart valve scaffolds prepared according to procedures currently considered for clinical use might contain significant amount of surfactant. Hindawi 2017 2017-06-06 /pmc/articles/PMC5476881/ /pubmed/28676861 http://dx.doi.org/10.1155/2017/9274135 Text en Copyright © 2017 Monica Dettin et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dettin, Monica
Zamuner, Annj
Naso, Filippo
Monteleone, Antonella
Spina, Michele
Gerosa, Gino
Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves
title Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves
title_full Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves
title_fullStr Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves
title_full_unstemmed Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves
title_short Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves
title_sort natural scaffolds for regenerative medicine: direct determination of detergents entrapped in decellularized heart valves
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476881/
https://www.ncbi.nlm.nih.gov/pubmed/28676861
http://dx.doi.org/10.1155/2017/9274135
work_keys_str_mv AT dettinmonica naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves
AT zamunerannj naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves
AT nasofilippo naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves
AT monteleoneantonella naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves
AT spinamichele naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves
AT gerosagino naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves