Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves
The increasing urgency for replacement of pathological heart valves is a major stimulus for research on alternatives to glutaraldehyde-treated grafts. New xenogeneic acellular heart valve substitutes that can be repopulated by host cells are currently under investigation. Anionic surfactants, includ...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476881/ https://www.ncbi.nlm.nih.gov/pubmed/28676861 http://dx.doi.org/10.1155/2017/9274135 |
_version_ | 1783244685023641600 |
---|---|
author | Dettin, Monica Zamuner, Annj Naso, Filippo Monteleone, Antonella Spina, Michele Gerosa, Gino |
author_facet | Dettin, Monica Zamuner, Annj Naso, Filippo Monteleone, Antonella Spina, Michele Gerosa, Gino |
author_sort | Dettin, Monica |
collection | PubMed |
description | The increasing urgency for replacement of pathological heart valves is a major stimulus for research on alternatives to glutaraldehyde-treated grafts. New xenogeneic acellular heart valve substitutes that can be repopulated by host cells are currently under investigation. Anionic surfactants, including bile acids, have been widely used to eliminate the resident cell components chiefly responsible for the immunogenicity of the tissue, even if detergent toxicity might present limitations to the survival and/or functional expression of the repopulating cells. To date, the determination of residual detergent has been carried out almost exclusively on the washings following cell removal procedures. Here, a novel HPLC-based procedure is proposed for the direct quantification of detergent (cholate, deoxycholate, and taurodeoxycholate) residues entrapped in the scaffold of decellularized porcine aortic and pulmonary valves. The method was demonstrated to be sensitive, reproducible, and extendable to different types of detergent. This assessment also revealed that cell-depleted heart valve scaffolds prepared according to procedures currently considered for clinical use might contain significant amount of surfactant. |
format | Online Article Text |
id | pubmed-5476881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-54768812017-07-04 Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves Dettin, Monica Zamuner, Annj Naso, Filippo Monteleone, Antonella Spina, Michele Gerosa, Gino Biomed Res Int Research Article The increasing urgency for replacement of pathological heart valves is a major stimulus for research on alternatives to glutaraldehyde-treated grafts. New xenogeneic acellular heart valve substitutes that can be repopulated by host cells are currently under investigation. Anionic surfactants, including bile acids, have been widely used to eliminate the resident cell components chiefly responsible for the immunogenicity of the tissue, even if detergent toxicity might present limitations to the survival and/or functional expression of the repopulating cells. To date, the determination of residual detergent has been carried out almost exclusively on the washings following cell removal procedures. Here, a novel HPLC-based procedure is proposed for the direct quantification of detergent (cholate, deoxycholate, and taurodeoxycholate) residues entrapped in the scaffold of decellularized porcine aortic and pulmonary valves. The method was demonstrated to be sensitive, reproducible, and extendable to different types of detergent. This assessment also revealed that cell-depleted heart valve scaffolds prepared according to procedures currently considered for clinical use might contain significant amount of surfactant. Hindawi 2017 2017-06-06 /pmc/articles/PMC5476881/ /pubmed/28676861 http://dx.doi.org/10.1155/2017/9274135 Text en Copyright © 2017 Monica Dettin et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dettin, Monica Zamuner, Annj Naso, Filippo Monteleone, Antonella Spina, Michele Gerosa, Gino Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves |
title | Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves |
title_full | Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves |
title_fullStr | Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves |
title_full_unstemmed | Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves |
title_short | Natural Scaffolds for Regenerative Medicine: Direct Determination of Detergents Entrapped in Decellularized Heart Valves |
title_sort | natural scaffolds for regenerative medicine: direct determination of detergents entrapped in decellularized heart valves |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476881/ https://www.ncbi.nlm.nih.gov/pubmed/28676861 http://dx.doi.org/10.1155/2017/9274135 |
work_keys_str_mv | AT dettinmonica naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves AT zamunerannj naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves AT nasofilippo naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves AT monteleoneantonella naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves AT spinamichele naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves AT gerosagino naturalscaffoldsforregenerativemedicinedirectdeterminationofdetergentsentrappedindecellularizedheartvalves |