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A multiplex primer design algorithm for target amplification of continuous genomic regions

BACKGROUND: Targeted Next Generation Sequencing (NGS) assays are cost-efficient and reliable alternatives to Sanger sequencing. For sequencing of very large set of genes, the target enrichment approach is suitable. However, for smaller genomic regions, the target amplification method is more efficie...

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Detalles Bibliográficos
Autores principales: Ozturk, Ahmet Rasit, Can, Tolga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5477098/
https://www.ncbi.nlm.nih.gov/pubmed/28629316
http://dx.doi.org/10.1186/s12859-017-1716-7