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Single‐molecule mechanochemical characterization of E. coli pol III core catalytic activity

Pol III core is the three‐subunit subassembly of the E. coli replicative DNA polymerase III holoenzyme. It contains the catalytic polymerase subunit α, the 3′ → 5′ proofreading exonuclease ε, and a subunit of unknown function, θ. We employ optical tweezers to characterize pol III core activity on a...

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Detalles Bibliográficos
Autores principales: Naufer, M. Nabuan, Murison, David A., Rouzina, Ioulia, Beuning, Penny J., Williams, Mark C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5477539/
https://www.ncbi.nlm.nih.gov/pubmed/28263430
http://dx.doi.org/10.1002/pro.3152