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Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase

The range of ATP concentrations that can be measured with a fluorescent reagentless biosensor for ATP has been increased by modulating its affinity for this analyte. The ATP biosensor is an adduct of two tetramethylrhodamines with MatB from Rhodopseudomonas palustris. Mutations were introduced into...

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Detalles Bibliográficos
Autores principales: Vancraenenbroeck, Renée, Kunzelmann, Simone, Webb, Martin R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479551/
https://www.ncbi.nlm.nih.gov/pubmed/28636641
http://dx.doi.org/10.1371/journal.pone.0179547