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Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase
The range of ATP concentrations that can be measured with a fluorescent reagentless biosensor for ATP has been increased by modulating its affinity for this analyte. The ATP biosensor is an adduct of two tetramethylrhodamines with MatB from Rhodopseudomonas palustris. Mutations were introduced into...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479551/ https://www.ncbi.nlm.nih.gov/pubmed/28636641 http://dx.doi.org/10.1371/journal.pone.0179547 |