His-FLAG Tag as a Fusion Partner of Glycosylated Human Interferon-Gamma and Its Mutant: Gain or Loss?

In order to obtain glycosylated human interferon-gamma (hIFNγ) and its highly prone to aggregation mutant K88Q, a secretory expression in insect cells was employed. To facilitate recombinant proteins purification, detection, and stability the baculovirus expression vectors were constructed to bear N...

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Detalles Bibliográficos
Autores principales: Krachmarova, Elena, Tileva, Milena, Lilkova, Elena, Petkov, Peicho, Maskos, Klaus, Ilieva, Nevena, Ivanov, Ivan, Litov, Leandar, Nacheva, Genoveva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5480026/
https://www.ncbi.nlm.nih.gov/pubmed/28685146
http://dx.doi.org/10.1155/2017/3018608

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5480026/
https://www.ncbi.nlm.nih.gov/pubmed/28685146
http://dx.doi.org/10.1155/2017/3018608

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