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Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice
A decline in the regenerative capacity of adult stem cells with aging is well documented. As a result of this decline, the efficacy of autologous stem cell therapies is likely to decline with increasing donor age. In these cases, strategies to restore the function of aged stem cells would have clini...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5480862/ https://www.ncbi.nlm.nih.gov/pubmed/28640861 http://dx.doi.org/10.1371/journal.pone.0179270 |
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author | Proto, Jonathan D. Lu, Aiping Dorronsoro, Akaitz Scibetta, Alex Robbins, Paul D. Niedernhofer, Laura J. Huard, Johnny |
author_facet | Proto, Jonathan D. Lu, Aiping Dorronsoro, Akaitz Scibetta, Alex Robbins, Paul D. Niedernhofer, Laura J. Huard, Johnny |
author_sort | Proto, Jonathan D. |
collection | PubMed |
description | A decline in the regenerative capacity of adult stem cells with aging is well documented. As a result of this decline, the efficacy of autologous stem cell therapies is likely to decline with increasing donor age. In these cases, strategies to restore the function of aged stem cells would have clinical utility. Globally, the transcription factor NF-κB is up-regulated in aged tissues. Given the negative role that NF-κB plays in myogenesis, we investigated whether the age-related decline in the function of muscle-derived stem/progenitor cells (MDSPCs) could be improved by inhibition of NF-κB. Herein, we demonstrate that pharmacologic or genetic inhibition of NF-κB activation increases myogenic differentiation and improves resistance to oxidative stress. Our results suggest that MDSPC “aging” may be reversible, and that pharmacologic targeting of pathways such as NF-κB may enhance the efficacy of cell-based therapies. |
format | Online Article Text |
id | pubmed-5480862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54808622017-07-05 Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice Proto, Jonathan D. Lu, Aiping Dorronsoro, Akaitz Scibetta, Alex Robbins, Paul D. Niedernhofer, Laura J. Huard, Johnny PLoS One Research Article A decline in the regenerative capacity of adult stem cells with aging is well documented. As a result of this decline, the efficacy of autologous stem cell therapies is likely to decline with increasing donor age. In these cases, strategies to restore the function of aged stem cells would have clinical utility. Globally, the transcription factor NF-κB is up-regulated in aged tissues. Given the negative role that NF-κB plays in myogenesis, we investigated whether the age-related decline in the function of muscle-derived stem/progenitor cells (MDSPCs) could be improved by inhibition of NF-κB. Herein, we demonstrate that pharmacologic or genetic inhibition of NF-κB activation increases myogenic differentiation and improves resistance to oxidative stress. Our results suggest that MDSPC “aging” may be reversible, and that pharmacologic targeting of pathways such as NF-κB may enhance the efficacy of cell-based therapies. Public Library of Science 2017-06-22 /pmc/articles/PMC5480862/ /pubmed/28640861 http://dx.doi.org/10.1371/journal.pone.0179270 Text en © 2017 Proto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Proto, Jonathan D. Lu, Aiping Dorronsoro, Akaitz Scibetta, Alex Robbins, Paul D. Niedernhofer, Laura J. Huard, Johnny Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice |
title | Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice |
title_full | Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice |
title_fullStr | Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice |
title_full_unstemmed | Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice |
title_short | Inhibition of NF-κB improves the stress resistance and myogenic differentiation of MDSPCs isolated from naturally aged mice |
title_sort | inhibition of nf-κb improves the stress resistance and myogenic differentiation of mdspcs isolated from naturally aged mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5480862/ https://www.ncbi.nlm.nih.gov/pubmed/28640861 http://dx.doi.org/10.1371/journal.pone.0179270 |
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