A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish

Clustered regularly interspaced palindromic repeats (CRISPR)/Cas-based adaptive immunity against pathogens in bacteria has been adapted for genome editing and applied in zebrafish (Danio rerio) to generate frameshift mutations in protein-coding genes. Although there are methods to detect, quantify a...

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Detalles Bibliográficos
Autores principales: Prykhozhij, Sergey V., Steele, Shelby L., Razaghi, Babak, Berman, Jason N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Resource Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483001/
https://www.ncbi.nlm.nih.gov/pubmed/28280001
http://dx.doi.org/10.1242/dmm.026765

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483001/
https://www.ncbi.nlm.nih.gov/pubmed/28280001
http://dx.doi.org/10.1242/dmm.026765

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