In vitro synthesis of uniform poly(dG)–poly(dC) by Klenow exo(−) fragment of polymerase I

In this paper, we describe a production procedure of the one-to-one double helical complex of poly(dG)–poly(dC), characterized by a well-defined length (up to 10 kb) and narrow size distribution of molecules. Direct evidence of strands slippage during poly(dG)–poly(dC) synthesis by Klenow exo(−) fra...

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Detalles Bibliográficos
Autores principales: Kotlyar, Alexander B., Borovok, Natalia, Molotsky, Tatiana, Fadeev, Ludmila, Gozin, Michael
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548336/
https://www.ncbi.nlm.nih.gov/pubmed/15673713
http://dx.doi.org/10.1093/nar/gki178

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548336/
https://www.ncbi.nlm.nih.gov/pubmed/15673713
http://dx.doi.org/10.1093/nar/gki178

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