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In vitro synthesis of uniform poly(dG)–poly(dC) by Klenow exo(−) fragment of polymerase I
In this paper, we describe a production procedure of the one-to-one double helical complex of poly(dG)–poly(dC), characterized by a well-defined length (up to 10 kb) and narrow size distribution of molecules. Direct evidence of strands slippage during poly(dG)–poly(dC) synthesis by Klenow exo(−) fra...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548336/ https://www.ncbi.nlm.nih.gov/pubmed/15673713 http://dx.doi.org/10.1093/nar/gki178 |