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Novel mobilizable prokaryotic two-hybrid system vectors for high-throughput protein interaction mapping in Escherichia coli by bacterial conjugation

Since its initial description, the yeast two-hybrid (Y2H) system has been widely used for the detection and analysis of protein–protein interactions. Mating-based strategies have been developed permitting its application for automated proteomic interaction mapping projects using both exhaustive and...

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Detalles Bibliográficos
Autores principales: Clarke, Paul, Cuív, Páraic Ó, O'Connell, Michael
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548371/
https://www.ncbi.nlm.nih.gov/pubmed/15687376
http://dx.doi.org/10.1093/nar/gni011