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Exonuclease III protection assay with FRET probe for detecting DNA-binding proteins

We describe a new method for the assay of sequence-specific DNA-binding proteins in this paper. In this method, the sensitive fluorescence resonance energy transfer (FRET) technology is combined with the common DNA footprinting assay in order to develop a simple, rapid and high-throughput approach f...

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Detalles Bibliográficos
Autores principales: Wang, Jinke, Li, Tongxiang, Guo, Xiaoying, Lu, Zuhong
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548379/
https://www.ncbi.nlm.nih.gov/pubmed/15687381
http://dx.doi.org/10.1093/nar/gni021