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A Simple Phosphate-Buffered-Saline-Based Extraction Method Improves Specificity of HIV Viral Load Monitoring Using Dried Blood Spots

Although Roche COBAS Ampliprep/COBAS TaqMan (CAP/CTM) systems are widely used in sub-Saharan Africa for early infant diagnosis of HIV from dried blood spots (DBS), viral load monitoring with this system is not practical due to nonspecific extraction of both cell-free and cell-associated viral nuclei...

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Detalles Bibliográficos
Autores principales: Makadzange, A. Tariro, Boyd, F. Kathryn, Chimukangara, Benjamin, Masimirembwa, Collen, Katzenstein, David, Ndhlovu, Chiratidzo E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483919/
https://www.ncbi.nlm.nih.gov/pubmed/28468852
http://dx.doi.org/10.1128/JCM.00176-17