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5-Aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga Porphyridium purpureum

BACKGROUND: The microalga Porphyridium purpureum within Rhodophyta abundantly produces several valuable proteins, polysaccharides, pigments and long-chain polyunsaturated fatty acid; it is especially effective in accumulating arachidonic acid (ARA). However, this high ARA yield is always achieved in...

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Autores principales: Jiao, Kailin, Chang, Jingyu, Zeng, Xianhai, Ng, I-Son, Xiao, Zongyuan, Sun, Yong, Tang, Xing, Lin, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485562/
https://www.ncbi.nlm.nih.gov/pubmed/28670342
http://dx.doi.org/10.1186/s13068-017-0855-4
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author Jiao, Kailin
Chang, Jingyu
Zeng, Xianhai
Ng, I-Son
Xiao, Zongyuan
Sun, Yong
Tang, Xing
Lin, Lu
author_facet Jiao, Kailin
Chang, Jingyu
Zeng, Xianhai
Ng, I-Son
Xiao, Zongyuan
Sun, Yong
Tang, Xing
Lin, Lu
author_sort Jiao, Kailin
collection PubMed
description BACKGROUND: The microalga Porphyridium purpureum within Rhodophyta abundantly produces several valuable proteins, polysaccharides, pigments and long-chain polyunsaturated fatty acid; it is especially effective in accumulating arachidonic acid (ARA). However, this high ARA yield is always achieved in conditions unfavourable for cell growth. In this study, we present a method for obtaining desirable ARA levels from P. purpureum while simultaneously promoting cell growth using appropriate concentrations of the growth hormone 5-Aminolevulinic acid (5-ALA). RESULTS: Both the biomass and the ARA content of P. purpureum were enhanced by stimulation with 20 mg/L 5-ALA, leading to an optimal ARA yield of 170.32 mg/L—a 70.82% increase compared with control conditions. This ARA yield is the highest ever reported for microalgae. Based on variations in the fatty acid composition, total lipids, total proteins, total carbohydrates and pigment content during the cultivation period, we propose that the accumulation of ARA stimulated by 5-ALA occurs at the expense of other UFAs and total proteins, which may be related to decreased zeaxanthin. Lipidomic analysis revealed that triacylglycerols (TAGs) accounted for 47.5 ± 3.6% of all detected lipids, followed by phosphatidylglycerol (PG) and digalactosyldiacylglycerol (DGDG). As the levels of the most abundant TAGs increased under 5-ALA promotion and because 78.1 ± 3.4% (by weight) of detected TAG-branched chains contained ARA, the increase of ARA was mainly caused by TAG accumulation. CONCLUSIONS: This work demonstrated a simple and effective strategy to promote both biomass and ARA yield in P. purpureum by introducing a small amount of 5-ALA. These results are helpful for understanding the microalgae metabolic pathways affected by phytohormones and for guiding the development of bioproducts from microalgae.
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spelling pubmed-54855622017-06-30 5-Aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga Porphyridium purpureum Jiao, Kailin Chang, Jingyu Zeng, Xianhai Ng, I-Son Xiao, Zongyuan Sun, Yong Tang, Xing Lin, Lu Biotechnol Biofuels Research BACKGROUND: The microalga Porphyridium purpureum within Rhodophyta abundantly produces several valuable proteins, polysaccharides, pigments and long-chain polyunsaturated fatty acid; it is especially effective in accumulating arachidonic acid (ARA). However, this high ARA yield is always achieved in conditions unfavourable for cell growth. In this study, we present a method for obtaining desirable ARA levels from P. purpureum while simultaneously promoting cell growth using appropriate concentrations of the growth hormone 5-Aminolevulinic acid (5-ALA). RESULTS: Both the biomass and the ARA content of P. purpureum were enhanced by stimulation with 20 mg/L 5-ALA, leading to an optimal ARA yield of 170.32 mg/L—a 70.82% increase compared with control conditions. This ARA yield is the highest ever reported for microalgae. Based on variations in the fatty acid composition, total lipids, total proteins, total carbohydrates and pigment content during the cultivation period, we propose that the accumulation of ARA stimulated by 5-ALA occurs at the expense of other UFAs and total proteins, which may be related to decreased zeaxanthin. Lipidomic analysis revealed that triacylglycerols (TAGs) accounted for 47.5 ± 3.6% of all detected lipids, followed by phosphatidylglycerol (PG) and digalactosyldiacylglycerol (DGDG). As the levels of the most abundant TAGs increased under 5-ALA promotion and because 78.1 ± 3.4% (by weight) of detected TAG-branched chains contained ARA, the increase of ARA was mainly caused by TAG accumulation. CONCLUSIONS: This work demonstrated a simple and effective strategy to promote both biomass and ARA yield in P. purpureum by introducing a small amount of 5-ALA. These results are helpful for understanding the microalgae metabolic pathways affected by phytohormones and for guiding the development of bioproducts from microalgae. BioMed Central 2017-06-26 /pmc/articles/PMC5485562/ /pubmed/28670342 http://dx.doi.org/10.1186/s13068-017-0855-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Jiao, Kailin
Chang, Jingyu
Zeng, Xianhai
Ng, I-Son
Xiao, Zongyuan
Sun, Yong
Tang, Xing
Lin, Lu
5-Aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga Porphyridium purpureum
title 5-Aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga Porphyridium purpureum
title_full 5-Aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga Porphyridium purpureum
title_fullStr 5-Aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga Porphyridium purpureum
title_full_unstemmed 5-Aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga Porphyridium purpureum
title_short 5-Aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga Porphyridium purpureum
title_sort 5-aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga porphyridium purpureum
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485562/
https://www.ncbi.nlm.nih.gov/pubmed/28670342
http://dx.doi.org/10.1186/s13068-017-0855-4
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