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Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging

A kind of fluorescent/phosphorescent dual-emissive conjugated polyelectrolyte has been prepared by introducing phosphorescent platinum(ii) porphyrin (O(2)-sensitive) into a fluorene-based conjugated polyelectrolyte (O(2)-insensitive), which can form ultrasmall conjugated polymer dots (FP-Pdots) in t...

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Autores principales: Zhao, Qiang, Zhou, Xiaobo, Cao, Tianye, Zhang, Kenneth Yin, Yang, Lijuan, Liu, Shujuan, Liang, Hua, Yang, Huiran, Li, Fuyou, Huang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485888/
https://www.ncbi.nlm.nih.gov/pubmed/28694947
http://dx.doi.org/10.1039/c4sc03062a
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author Zhao, Qiang
Zhou, Xiaobo
Cao, Tianye
Zhang, Kenneth Yin
Yang, Lijuan
Liu, Shujuan
Liang, Hua
Yang, Huiran
Li, Fuyou
Huang, Wei
author_facet Zhao, Qiang
Zhou, Xiaobo
Cao, Tianye
Zhang, Kenneth Yin
Yang, Lijuan
Liu, Shujuan
Liang, Hua
Yang, Huiran
Li, Fuyou
Huang, Wei
author_sort Zhao, Qiang
collection PubMed
description A kind of fluorescent/phosphorescent dual-emissive conjugated polyelectrolyte has been prepared by introducing phosphorescent platinum(ii) porphyrin (O(2)-sensitive) into a fluorene-based conjugated polyelectrolyte (O(2)-insensitive), which can form ultrasmall conjugated polymer dots (FP-Pdots) in the phosphate buffer solution (PBS) via self-assembly caused by their amphiphilic structures with hydrophobic backbones and hydrophilic side chains. These FP-Pdots can exhibit an excellent ratiometric luminescence response to O(2) content with high reliability and full reversibility for measuring oxygen levels, and the excellent intracellular ratiometric O(2) sensing properties of the FP-Pdots nanoprobe have also been confirmed by the evident change in the I (red)/I (blue) ratio values in living cells cultured at different O(2) concentrations. To confirm the reliability of the O(2) sensing measurements of the FP-Pdots nanoprobe, O(2) quenching experiments based on lifetime measurements of phosphorescence from Pt(ii) porphyrin moieties have also been carried out. Utilizing the sensitivity of the long phosphorescence lifetime from Pt(ii) porphyrins to oxygen, the FP-Pdots have been successfully applied in time-resolved luminescence imaging of intracellular O(2) levels, including photoluminescence lifetime imaging and time-gated luminescence imaging, which will evidently improve the sensing sensitivity and reliability. Finally, in vivo oxygen sensing experiments were successfully performed by luminescence imaging of tumor hypoxia in nude mice.
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spelling pubmed-54858882017-07-10 Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging Zhao, Qiang Zhou, Xiaobo Cao, Tianye Zhang, Kenneth Yin Yang, Lijuan Liu, Shujuan Liang, Hua Yang, Huiran Li, Fuyou Huang, Wei Chem Sci Chemistry A kind of fluorescent/phosphorescent dual-emissive conjugated polyelectrolyte has been prepared by introducing phosphorescent platinum(ii) porphyrin (O(2)-sensitive) into a fluorene-based conjugated polyelectrolyte (O(2)-insensitive), which can form ultrasmall conjugated polymer dots (FP-Pdots) in the phosphate buffer solution (PBS) via self-assembly caused by their amphiphilic structures with hydrophobic backbones and hydrophilic side chains. These FP-Pdots can exhibit an excellent ratiometric luminescence response to O(2) content with high reliability and full reversibility for measuring oxygen levels, and the excellent intracellular ratiometric O(2) sensing properties of the FP-Pdots nanoprobe have also been confirmed by the evident change in the I (red)/I (blue) ratio values in living cells cultured at different O(2) concentrations. To confirm the reliability of the O(2) sensing measurements of the FP-Pdots nanoprobe, O(2) quenching experiments based on lifetime measurements of phosphorescence from Pt(ii) porphyrin moieties have also been carried out. Utilizing the sensitivity of the long phosphorescence lifetime from Pt(ii) porphyrins to oxygen, the FP-Pdots have been successfully applied in time-resolved luminescence imaging of intracellular O(2) levels, including photoluminescence lifetime imaging and time-gated luminescence imaging, which will evidently improve the sensing sensitivity and reliability. Finally, in vivo oxygen sensing experiments were successfully performed by luminescence imaging of tumor hypoxia in nude mice. Royal Society of Chemistry 2015-03-01 2015-01-12 /pmc/articles/PMC5485888/ /pubmed/28694947 http://dx.doi.org/10.1039/c4sc03062a Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Zhao, Qiang
Zhou, Xiaobo
Cao, Tianye
Zhang, Kenneth Yin
Yang, Lijuan
Liu, Shujuan
Liang, Hua
Yang, Huiran
Li, Fuyou
Huang, Wei
Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
title Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
title_full Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
title_fullStr Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
title_full_unstemmed Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
title_short Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
title_sort fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485888/
https://www.ncbi.nlm.nih.gov/pubmed/28694947
http://dx.doi.org/10.1039/c4sc03062a
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