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Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
A kind of fluorescent/phosphorescent dual-emissive conjugated polyelectrolyte has been prepared by introducing phosphorescent platinum(ii) porphyrin (O(2)-sensitive) into a fluorene-based conjugated polyelectrolyte (O(2)-insensitive), which can form ultrasmall conjugated polymer dots (FP-Pdots) in t...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485888/ https://www.ncbi.nlm.nih.gov/pubmed/28694947 http://dx.doi.org/10.1039/c4sc03062a |
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author | Zhao, Qiang Zhou, Xiaobo Cao, Tianye Zhang, Kenneth Yin Yang, Lijuan Liu, Shujuan Liang, Hua Yang, Huiran Li, Fuyou Huang, Wei |
author_facet | Zhao, Qiang Zhou, Xiaobo Cao, Tianye Zhang, Kenneth Yin Yang, Lijuan Liu, Shujuan Liang, Hua Yang, Huiran Li, Fuyou Huang, Wei |
author_sort | Zhao, Qiang |
collection | PubMed |
description | A kind of fluorescent/phosphorescent dual-emissive conjugated polyelectrolyte has been prepared by introducing phosphorescent platinum(ii) porphyrin (O(2)-sensitive) into a fluorene-based conjugated polyelectrolyte (O(2)-insensitive), which can form ultrasmall conjugated polymer dots (FP-Pdots) in the phosphate buffer solution (PBS) via self-assembly caused by their amphiphilic structures with hydrophobic backbones and hydrophilic side chains. These FP-Pdots can exhibit an excellent ratiometric luminescence response to O(2) content with high reliability and full reversibility for measuring oxygen levels, and the excellent intracellular ratiometric O(2) sensing properties of the FP-Pdots nanoprobe have also been confirmed by the evident change in the I (red)/I (blue) ratio values in living cells cultured at different O(2) concentrations. To confirm the reliability of the O(2) sensing measurements of the FP-Pdots nanoprobe, O(2) quenching experiments based on lifetime measurements of phosphorescence from Pt(ii) porphyrin moieties have also been carried out. Utilizing the sensitivity of the long phosphorescence lifetime from Pt(ii) porphyrins to oxygen, the FP-Pdots have been successfully applied in time-resolved luminescence imaging of intracellular O(2) levels, including photoluminescence lifetime imaging and time-gated luminescence imaging, which will evidently improve the sensing sensitivity and reliability. Finally, in vivo oxygen sensing experiments were successfully performed by luminescence imaging of tumor hypoxia in nude mice. |
format | Online Article Text |
id | pubmed-5485888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-54858882017-07-10 Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging Zhao, Qiang Zhou, Xiaobo Cao, Tianye Zhang, Kenneth Yin Yang, Lijuan Liu, Shujuan Liang, Hua Yang, Huiran Li, Fuyou Huang, Wei Chem Sci Chemistry A kind of fluorescent/phosphorescent dual-emissive conjugated polyelectrolyte has been prepared by introducing phosphorescent platinum(ii) porphyrin (O(2)-sensitive) into a fluorene-based conjugated polyelectrolyte (O(2)-insensitive), which can form ultrasmall conjugated polymer dots (FP-Pdots) in the phosphate buffer solution (PBS) via self-assembly caused by their amphiphilic structures with hydrophobic backbones and hydrophilic side chains. These FP-Pdots can exhibit an excellent ratiometric luminescence response to O(2) content with high reliability and full reversibility for measuring oxygen levels, and the excellent intracellular ratiometric O(2) sensing properties of the FP-Pdots nanoprobe have also been confirmed by the evident change in the I (red)/I (blue) ratio values in living cells cultured at different O(2) concentrations. To confirm the reliability of the O(2) sensing measurements of the FP-Pdots nanoprobe, O(2) quenching experiments based on lifetime measurements of phosphorescence from Pt(ii) porphyrin moieties have also been carried out. Utilizing the sensitivity of the long phosphorescence lifetime from Pt(ii) porphyrins to oxygen, the FP-Pdots have been successfully applied in time-resolved luminescence imaging of intracellular O(2) levels, including photoluminescence lifetime imaging and time-gated luminescence imaging, which will evidently improve the sensing sensitivity and reliability. Finally, in vivo oxygen sensing experiments were successfully performed by luminescence imaging of tumor hypoxia in nude mice. Royal Society of Chemistry 2015-03-01 2015-01-12 /pmc/articles/PMC5485888/ /pubmed/28694947 http://dx.doi.org/10.1039/c4sc03062a Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Zhao, Qiang Zhou, Xiaobo Cao, Tianye Zhang, Kenneth Yin Yang, Lijuan Liu, Shujuan Liang, Hua Yang, Huiran Li, Fuyou Huang, Wei Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging |
title | Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
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title_full | Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
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title_fullStr | Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
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title_full_unstemmed | Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
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title_short | Fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging
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title_sort | fluorescent/phosphorescent dual-emissive conjugated polymer dots for hypoxia bioimaging |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485888/ https://www.ncbi.nlm.nih.gov/pubmed/28694947 http://dx.doi.org/10.1039/c4sc03062a |
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