Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties

Three different ionene polymers with varying quaternary ammonium moieties were used as a proof of concept for the formulation of antisense oligonucleotides, which are capable of inhibiting Renilla luciferase messenger ribonucleic acid (mRNA). Cationic vesicles, consisting of cationic polymer, antise...

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Autores principales: Mayr, Judith, Grijalvo, Santiago, Bachl, Jürgen, Pons, Ramon, Eritja, Ramon, Díaz Díaz, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485963/
https://www.ncbi.nlm.nih.gov/pubmed/28587106
http://dx.doi.org/10.3390/ijms18061139
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author Mayr, Judith
Grijalvo, Santiago
Bachl, Jürgen
Pons, Ramon
Eritja, Ramon
Díaz Díaz, David
author_facet Mayr, Judith
Grijalvo, Santiago
Bachl, Jürgen
Pons, Ramon
Eritja, Ramon
Díaz Díaz, David
author_sort Mayr, Judith
collection PubMed
description Three different ionene polymers with varying quaternary ammonium moieties were used as a proof of concept for the formulation of antisense oligonucleotides, which are capable of inhibiting Renilla luciferase messenger ribonucleic acid (mRNA). Cationic vesicles, consisting of cationic polymer, antisense oligonucleotide (Luc) and non-ionic surfactant polysorbate 80, were investigated regarding their ζ potential, cytotoxicity and transfection efficiency. Deoxyribonucleic acid- (DNA) forming complexes in the presence of cationic vesicles were also investigated in terms of small-angle X-ray scattering (SAXS). The studied cationic vesicles showed very little, if any, toxicity against HeLa cells. Transfection abilities proved to vary strongly depending on the present quaternary ammonium moiety.
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spelling pubmed-54859632017-06-29 Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties Mayr, Judith Grijalvo, Santiago Bachl, Jürgen Pons, Ramon Eritja, Ramon Díaz Díaz, David Int J Mol Sci Communication Three different ionene polymers with varying quaternary ammonium moieties were used as a proof of concept for the formulation of antisense oligonucleotides, which are capable of inhibiting Renilla luciferase messenger ribonucleic acid (mRNA). Cationic vesicles, consisting of cationic polymer, antisense oligonucleotide (Luc) and non-ionic surfactant polysorbate 80, were investigated regarding their ζ potential, cytotoxicity and transfection efficiency. Deoxyribonucleic acid- (DNA) forming complexes in the presence of cationic vesicles were also investigated in terms of small-angle X-ray scattering (SAXS). The studied cationic vesicles showed very little, if any, toxicity against HeLa cells. Transfection abilities proved to vary strongly depending on the present quaternary ammonium moiety. MDPI 2017-05-26 /pmc/articles/PMC5485963/ /pubmed/28587106 http://dx.doi.org/10.3390/ijms18061139 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Mayr, Judith
Grijalvo, Santiago
Bachl, Jürgen
Pons, Ramon
Eritja, Ramon
Díaz Díaz, David
Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties
title Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties
title_full Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties
title_fullStr Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties
title_full_unstemmed Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties
title_short Transfection of Antisense Oligonucleotides Mediated by Cationic Vesicles Based on Non-Ionic Surfactant and Polycations Bearing Quaternary Ammonium Moieties
title_sort transfection of antisense oligonucleotides mediated by cationic vesicles based on non-ionic surfactant and polycations bearing quaternary ammonium moieties
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485963/
https://www.ncbi.nlm.nih.gov/pubmed/28587106
http://dx.doi.org/10.3390/ijms18061139
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