EcoBLMcrX, a classical modification-dependent restriction enzyme in Escherichia coli B: Characterization in vivo and in vitro with a new approach to cleavage site determination

Here we characterize the modification-dependent restriction enzyme (MDE) EcoBLMcrX in vivo, in vitro and in its genomic environment. MDE cleavage of modified DNAs protects prokaryote populations from lethal infection by bacteriophage with highly modified DNA, and also stabilizes lineages by reducing...

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Detalles Bibliográficos
Autores principales: Fomenkov, Alexey, Sun, Zhiyi, Dila, Deborah K., Anton, Brian P., Roberts, Richard J., Raleigh, Elisabeth A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487053/
https://www.ncbi.nlm.nih.gov/pubmed/28654677
http://dx.doi.org/10.1371/journal.pone.0179853

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487053/
https://www.ncbi.nlm.nih.gov/pubmed/28654677
http://dx.doi.org/10.1371/journal.pone.0179853

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