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Click-PEGylation – A mobility shift approach to assess the redox state of cysteines in candidate proteins
The redox state of cysteine thiols is critical for protein function. Whereas cysteines play an important role in the maintenance of protein structure through the formation of internal disulfides, their nucleophilic thiol groups can become oxidatively modified in response to diverse redox challenges...
Autores principales: | van Leeuwen, Lucie A.G., Hinchy, Elizabeth C., Murphy, Michael P., Robb, Ellen L., Cochemé, Helena M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488967/ https://www.ncbi.nlm.nih.gov/pubmed/28366801 http://dx.doi.org/10.1016/j.freeradbiomed.2017.03.037 |
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