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Spectral insights into the transformation and distribution of CdSe quantum dots in microorganisms during food-chain transport

The discharge of engineered nanomaterials (ENMs) into environment is raising widespread concern not only due to their direction bio-toxicity but also their bio-concentration and bio-magnification through food web. However, the transformation and distribution of ENMs during food-chain transport are p...

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Autores principales: Tian, Li-Jiao, Peng, Yong, Chen, Dong-Liang, Ma, Jing-Yuan, Yu, Han-Qing, Li, Wen-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491517/
https://www.ncbi.nlm.nih.gov/pubmed/28663551
http://dx.doi.org/10.1038/s41598-017-04694-6
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author Tian, Li-Jiao
Peng, Yong
Chen, Dong-Liang
Ma, Jing-Yuan
Yu, Han-Qing
Li, Wen-Wei
author_facet Tian, Li-Jiao
Peng, Yong
Chen, Dong-Liang
Ma, Jing-Yuan
Yu, Han-Qing
Li, Wen-Wei
author_sort Tian, Li-Jiao
collection PubMed
description The discharge of engineered nanomaterials (ENMs) into environment is raising widespread concern not only due to their direction bio-toxicity but also their bio-concentration and bio-magnification through food web. However, the transformation and distribution of ENMs during food-chain transport are poorly understood, due to lack of accurate, reliable analytical methods. In this study, by using a suite of advanced spectrum techniques, we successfully tracked the distribution and biotransformation dynamics of CdSe quantum dots (QDs) during their transport from Shewanella onedensis to Caenorchabditis elegans in predation. Fluorescence microscopy and Raman mapping showed that the ingested QDs by C. elegans were located at the gut lumen and subcutaneous tissue, and were partially excreted from the nematode body over time. Micro-X-ray fluorescence (μ-XRF) spectroscopy and Se K-edge X-ray absorption fine structure (XAFS) results further revealed the changed distribution of Se element over time, and a shift in the major Se species from CdSe to Se(0) and Na(2)SeO(3) (IV). This work demonstrates the utility of advanced spectral techniques for characterizing QDs in vivo, and may facilitate a better understanding on the environmental transformation and fates of ENMs.
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spelling pubmed-54915172017-07-05 Spectral insights into the transformation and distribution of CdSe quantum dots in microorganisms during food-chain transport Tian, Li-Jiao Peng, Yong Chen, Dong-Liang Ma, Jing-Yuan Yu, Han-Qing Li, Wen-Wei Sci Rep Article The discharge of engineered nanomaterials (ENMs) into environment is raising widespread concern not only due to their direction bio-toxicity but also their bio-concentration and bio-magnification through food web. However, the transformation and distribution of ENMs during food-chain transport are poorly understood, due to lack of accurate, reliable analytical methods. In this study, by using a suite of advanced spectrum techniques, we successfully tracked the distribution and biotransformation dynamics of CdSe quantum dots (QDs) during their transport from Shewanella onedensis to Caenorchabditis elegans in predation. Fluorescence microscopy and Raman mapping showed that the ingested QDs by C. elegans were located at the gut lumen and subcutaneous tissue, and were partially excreted from the nematode body over time. Micro-X-ray fluorescence (μ-XRF) spectroscopy and Se K-edge X-ray absorption fine structure (XAFS) results further revealed the changed distribution of Se element over time, and a shift in the major Se species from CdSe to Se(0) and Na(2)SeO(3) (IV). This work demonstrates the utility of advanced spectral techniques for characterizing QDs in vivo, and may facilitate a better understanding on the environmental transformation and fates of ENMs. Nature Publishing Group UK 2017-06-29 /pmc/articles/PMC5491517/ /pubmed/28663551 http://dx.doi.org/10.1038/s41598-017-04694-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tian, Li-Jiao
Peng, Yong
Chen, Dong-Liang
Ma, Jing-Yuan
Yu, Han-Qing
Li, Wen-Wei
Spectral insights into the transformation and distribution of CdSe quantum dots in microorganisms during food-chain transport
title Spectral insights into the transformation and distribution of CdSe quantum dots in microorganisms during food-chain transport
title_full Spectral insights into the transformation and distribution of CdSe quantum dots in microorganisms during food-chain transport
title_fullStr Spectral insights into the transformation and distribution of CdSe quantum dots in microorganisms during food-chain transport
title_full_unstemmed Spectral insights into the transformation and distribution of CdSe quantum dots in microorganisms during food-chain transport
title_short Spectral insights into the transformation and distribution of CdSe quantum dots in microorganisms during food-chain transport
title_sort spectral insights into the transformation and distribution of cdse quantum dots in microorganisms during food-chain transport
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491517/
https://www.ncbi.nlm.nih.gov/pubmed/28663551
http://dx.doi.org/10.1038/s41598-017-04694-6
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