A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells

Here, we describe a novel method based on intronic MiMIC insertions described in Nagarkar-Jaiswal et al. (2015) to perform conditional gene inactivation in Drosophila. Mosaic analysis in Drosophila cannot be easily performed in post-mitotic cells. We therefore, therefore, developed Flip-Flop, a flip...

Descripción completa

Detalles Bibliográficos
Autores principales: Nagarkar-Jaiswal, Sonal, Manivannan, Sathiya N, Zuo, Zhongyuan, Bellen, Hugo J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Developmental Biology and Stem Cells
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493436/
https://www.ncbi.nlm.nih.gov/pubmed/28561736
http://dx.doi.org/10.7554/eLife.26420

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493436/
https://www.ncbi.nlm.nih.gov/pubmed/28561736
http://dx.doi.org/10.7554/eLife.26420

Ejemplares similares