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A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells
Here, we describe a novel method based on intronic MiMIC insertions described in Nagarkar-Jaiswal et al. (2015) to perform conditional gene inactivation in Drosophila. Mosaic analysis in Drosophila cannot be easily performed in post-mitotic cells. We therefore, therefore, developed Flip-Flop, a flip...
Autores principales: | Nagarkar-Jaiswal, Sonal, Manivannan, Sathiya N, Zuo, Zhongyuan, Bellen, Hugo J |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493436/ https://www.ncbi.nlm.nih.gov/pubmed/28561736 http://dx.doi.org/10.7554/eLife.26420 |
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