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Investigation of Mg(2+)- and temperature-dependent folding of the hairpin ribozyme by photo-crosslinking: effects of photo-crosslinker tether length and chemistry
We have used photo-crosslinking to investigate the structure and dynamics of four-way junction hairpin ribozyme constructs. Four phenylazide photo-crosslinkers were coupled to 2′-NH(2)-modified U+2 in the substrate and irradiated at different Mg(2+) concentrations and temperatures. Consistent with t...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549404/ https://www.ncbi.nlm.nih.gov/pubmed/15722480 http://dx.doi.org/10.1093/nar/gki237 |