Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA

Several genomic disorders are caused by an excessive number of DNA triplet repeats. We developed a DNA-templated reaction in which product formation occurs only when the number of repeats exceeds a threshold indicative for the outbreak of Chorea Huntington. The combined use of native chemical PNA li...

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Detalles Bibliográficos
Autores principales: Kern, Anika, Seitz, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494559/
https://www.ncbi.nlm.nih.gov/pubmed/28706635
http://dx.doi.org/10.1039/c4sc01974a
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author Kern, Anika
Seitz, Oliver
author_facet Kern, Anika
Seitz, Oliver
author_sort Kern, Anika
collection PubMed
description Several genomic disorders are caused by an excessive number of DNA triplet repeats. We developed a DNA-templated reaction in which product formation occurs only when the number of repeats exceeds a threshold indicative for the outbreak of Chorea Huntington. The combined use of native chemical PNA ligation and auxiliary DNA probes enabled reactions on templates obtained from human genomic DNA.
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spelling pubmed-54945592017-07-13 Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA Kern, Anika Seitz, Oliver Chem Sci Chemistry Several genomic disorders are caused by an excessive number of DNA triplet repeats. We developed a DNA-templated reaction in which product formation occurs only when the number of repeats exceeds a threshold indicative for the outbreak of Chorea Huntington. The combined use of native chemical PNA ligation and auxiliary DNA probes enabled reactions on templates obtained from human genomic DNA. Royal Society of Chemistry 2015-01-01 2014-09-16 /pmc/articles/PMC5494559/ /pubmed/28706635 http://dx.doi.org/10.1039/c4sc01974a Text en This journal is © The Royal Society of Chemistry 2014 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Kern, Anika
Seitz, Oliver
Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA
title Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA
title_full Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA
title_fullStr Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA
title_full_unstemmed Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA
title_short Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA
title_sort template-directed ligation on repetitive dna sequences: a chemical method to probe the length of huntington dna
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494559/
https://www.ncbi.nlm.nih.gov/pubmed/28706635
http://dx.doi.org/10.1039/c4sc01974a
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