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Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines

The present study investigated the effects of two major dietary fatty acid components, linoleic acid (LA) and elaidic acid (EA), on the antitumor effects of 5-fluorouracil (5-FU) in the LL2, CT26 and CMT93 mouse cancer cell lines. Concurrent treatment with LA and 5-FU elicited a decreased cell viabi...

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Autores principales: Tanabe, Eriko, Kitayoshi, Misaho, Fujii, Kiyomu, Ohmori, Hitoshi, Luo, Yi, Kadochi, Yui, Mori, Shiori, Fujiwara, Rina, Nishiguchi, Yukiko, Sasaki, Takamitsu, Kuniyasu, Hiroki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494753/
https://www.ncbi.nlm.nih.gov/pubmed/28693221
http://dx.doi.org/10.3892/ol.2017.6190
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author Tanabe, Eriko
Kitayoshi, Misaho
Fujii, Kiyomu
Ohmori, Hitoshi
Luo, Yi
Kadochi, Yui
Mori, Shiori
Fujiwara, Rina
Nishiguchi, Yukiko
Sasaki, Takamitsu
Kuniyasu, Hiroki
author_facet Tanabe, Eriko
Kitayoshi, Misaho
Fujii, Kiyomu
Ohmori, Hitoshi
Luo, Yi
Kadochi, Yui
Mori, Shiori
Fujiwara, Rina
Nishiguchi, Yukiko
Sasaki, Takamitsu
Kuniyasu, Hiroki
author_sort Tanabe, Eriko
collection PubMed
description The present study investigated the effects of two major dietary fatty acid components, linoleic acid (LA) and elaidic acid (EA), on the antitumor effects of 5-fluorouracil (5-FU) in the LL2, CT26 and CMT93 mouse cancer cell lines. Concurrent treatment with LA and 5-FU elicited a decreased cell viability compared with treatment with 5-FU alone. In addition, increased inhibition of growth was observed following concurrent treatment with EA and 5-FU. Sequential treatment of LA followed by 5-FU abrogated the anticancer effects of 5-FU, and treatment with EA followed by 5-FU increased cancer cell growth in addition to abrogating the anticancer effects of 5-FU. The expression of the stem cell markers CD133 and nucleostemin (NS) increased in all three cell lines treated concurrently with 5-FU and either LA or EA when compared with cells treated with 5-FU alone. Aldehyde dehydrogenase activity in the cancer stem cells (CSCs), in response to concurrent treatment with 5-FU and either LA or EA, was increased compared with 5-FU treatment alone. 5-FU inhibited the growth of CT26 tumors, but co-treatment with either LA or EA abrogated this effect. NS-positive CSCs were more abundant in CT26 tumors treated with 5-FU and either LA or EA compared with those treated with 5-FU alone. The results of the present study suggested that, rather than altering the sensitivity of cancer cells to 5-FU, LA and EA may promote the survival of CSCs. The results indicated that dietary composition during chemotherapy is an important issue.
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spelling pubmed-54947532017-07-07 Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines Tanabe, Eriko Kitayoshi, Misaho Fujii, Kiyomu Ohmori, Hitoshi Luo, Yi Kadochi, Yui Mori, Shiori Fujiwara, Rina Nishiguchi, Yukiko Sasaki, Takamitsu Kuniyasu, Hiroki Oncol Lett Articles The present study investigated the effects of two major dietary fatty acid components, linoleic acid (LA) and elaidic acid (EA), on the antitumor effects of 5-fluorouracil (5-FU) in the LL2, CT26 and CMT93 mouse cancer cell lines. Concurrent treatment with LA and 5-FU elicited a decreased cell viability compared with treatment with 5-FU alone. In addition, increased inhibition of growth was observed following concurrent treatment with EA and 5-FU. Sequential treatment of LA followed by 5-FU abrogated the anticancer effects of 5-FU, and treatment with EA followed by 5-FU increased cancer cell growth in addition to abrogating the anticancer effects of 5-FU. The expression of the stem cell markers CD133 and nucleostemin (NS) increased in all three cell lines treated concurrently with 5-FU and either LA or EA when compared with cells treated with 5-FU alone. Aldehyde dehydrogenase activity in the cancer stem cells (CSCs), in response to concurrent treatment with 5-FU and either LA or EA, was increased compared with 5-FU treatment alone. 5-FU inhibited the growth of CT26 tumors, but co-treatment with either LA or EA abrogated this effect. NS-positive CSCs were more abundant in CT26 tumors treated with 5-FU and either LA or EA compared with those treated with 5-FU alone. The results of the present study suggested that, rather than altering the sensitivity of cancer cells to 5-FU, LA and EA may promote the survival of CSCs. The results indicated that dietary composition during chemotherapy is an important issue. D.A. Spandidos 2017-07 2017-05-17 /pmc/articles/PMC5494753/ /pubmed/28693221 http://dx.doi.org/10.3892/ol.2017.6190 Text en Copyright: © Tanabe et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Tanabe, Eriko
Kitayoshi, Misaho
Fujii, Kiyomu
Ohmori, Hitoshi
Luo, Yi
Kadochi, Yui
Mori, Shiori
Fujiwara, Rina
Nishiguchi, Yukiko
Sasaki, Takamitsu
Kuniyasu, Hiroki
Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines
title Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines
title_full Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines
title_fullStr Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines
title_full_unstemmed Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines
title_short Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines
title_sort fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494753/
https://www.ncbi.nlm.nih.gov/pubmed/28693221
http://dx.doi.org/10.3892/ol.2017.6190
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