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Accumulation of RNA on chromatin disrupts heterochromatic silencing

Long noncoding RNAs (lncRNAs) play a conserved role in regulating gene expression, chromatin dynamics, and cell differentiation. They serve as a platform for RNA interference (RNAi)–mediated heterochromatin formation or DNA methylation in many eukaryotic organisms. We found in Schizosaccharomyces po...

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Autores principales: Brönner, Cornelia, Salvi, Luca, Zocco, Manuel, Ugolini, Ilaria, Halic, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5495069/
https://www.ncbi.nlm.nih.gov/pubmed/28404620
http://dx.doi.org/10.1101/gr.216986.116
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author Brönner, Cornelia
Salvi, Luca
Zocco, Manuel
Ugolini, Ilaria
Halic, Mario
author_facet Brönner, Cornelia
Salvi, Luca
Zocco, Manuel
Ugolini, Ilaria
Halic, Mario
author_sort Brönner, Cornelia
collection PubMed
description Long noncoding RNAs (lncRNAs) play a conserved role in regulating gene expression, chromatin dynamics, and cell differentiation. They serve as a platform for RNA interference (RNAi)–mediated heterochromatin formation or DNA methylation in many eukaryotic organisms. We found in Schizosaccharomyces pombe that heterochromatin is lost at transcribed regions in the absence of RNA degradation. We show that heterochromatic RNAs are retained on chromatin, form DNA:RNA hybrids, and need to be degraded by the Ccr4-Not complex or RNAi to maintain heterochromatic silencing. The Ccr4-Not complex is localized to chromatin independently of H3K9me and degrades chromatin-associated transcripts, which is required for transcriptional silencing. Overexpression of heterochromatic RNA, but not euchromatic RNA, leads to chromatin localization and loss of silencing of a distant ade6 reporter in wild-type cells. Our results demonstrate that chromatin-bound RNAs disrupt heterochromatin organization and need to be degraded in a process of heterochromatin formation.
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spelling pubmed-54950692018-01-01 Accumulation of RNA on chromatin disrupts heterochromatic silencing Brönner, Cornelia Salvi, Luca Zocco, Manuel Ugolini, Ilaria Halic, Mario Genome Res Research Long noncoding RNAs (lncRNAs) play a conserved role in regulating gene expression, chromatin dynamics, and cell differentiation. They serve as a platform for RNA interference (RNAi)–mediated heterochromatin formation or DNA methylation in many eukaryotic organisms. We found in Schizosaccharomyces pombe that heterochromatin is lost at transcribed regions in the absence of RNA degradation. We show that heterochromatic RNAs are retained on chromatin, form DNA:RNA hybrids, and need to be degraded by the Ccr4-Not complex or RNAi to maintain heterochromatic silencing. The Ccr4-Not complex is localized to chromatin independently of H3K9me and degrades chromatin-associated transcripts, which is required for transcriptional silencing. Overexpression of heterochromatic RNA, but not euchromatic RNA, leads to chromatin localization and loss of silencing of a distant ade6 reporter in wild-type cells. Our results demonstrate that chromatin-bound RNAs disrupt heterochromatin organization and need to be degraded in a process of heterochromatin formation. Cold Spring Harbor Laboratory Press 2017-07 /pmc/articles/PMC5495069/ /pubmed/28404620 http://dx.doi.org/10.1101/gr.216986.116 Text en © 2017 Brönner et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research
Brönner, Cornelia
Salvi, Luca
Zocco, Manuel
Ugolini, Ilaria
Halic, Mario
Accumulation of RNA on chromatin disrupts heterochromatic silencing
title Accumulation of RNA on chromatin disrupts heterochromatic silencing
title_full Accumulation of RNA on chromatin disrupts heterochromatic silencing
title_fullStr Accumulation of RNA on chromatin disrupts heterochromatic silencing
title_full_unstemmed Accumulation of RNA on chromatin disrupts heterochromatic silencing
title_short Accumulation of RNA on chromatin disrupts heterochromatic silencing
title_sort accumulation of rna on chromatin disrupts heterochromatic silencing
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5495069/
https://www.ncbi.nlm.nih.gov/pubmed/28404620
http://dx.doi.org/10.1101/gr.216986.116
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