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Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure
Dorsal closure (DC) is a developmental process in which two contralateral epithelial sheets migrate to seal a large hole in the dorsal ectoderm of the Drosophila embryo. Two signaling pathways act sequentially to orchestrate this dynamic morphogenetic process. First, c-Jun N-terminal kinase (JNK) si...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5495517/ https://www.ncbi.nlm.nih.gov/pubmed/28628612 http://dx.doi.org/10.1371/journal.pgen.1006860 |
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author | Kushnir, Tatyana Mezuman, Sharon Bar-Cohen, Shaked Lange, Rotem Paroush, Ze'ev Helman, Aharon |
author_facet | Kushnir, Tatyana Mezuman, Sharon Bar-Cohen, Shaked Lange, Rotem Paroush, Ze'ev Helman, Aharon |
author_sort | Kushnir, Tatyana |
collection | PubMed |
description | Dorsal closure (DC) is a developmental process in which two contralateral epithelial sheets migrate to seal a large hole in the dorsal ectoderm of the Drosophila embryo. Two signaling pathways act sequentially to orchestrate this dynamic morphogenetic process. First, c-Jun N-terminal kinase (JNK) signaling activity in the dorsal-most leading edge (LE) cells of the epidermis induces expression of decapentaplegic (dpp). Second, Dpp, a secreted TGF-β homolog, triggers cell shape changes in the adjacent, ventrally located lateral epidermis, that guide the morphogenetic movements and cell migration mandatory for DC. Here we uncover a cell non-autonomous requirement for the Epidermal growth factor receptor (Egfr) pathway in the lateral epidermis for sustained dpp expression in the LE. Specifically, we demonstrate that Egfr pathway activity in the lateral epidermis prevents expression of the gene scarface (scaf), encoding a secreted antagonist of JNK signaling. In embryos with compromised Egfr signaling, upregulated Scaf causes reduction of JNK activity in LE cells, thereby impeding completion of DC. Our results identify a new developmental role for Egfr signaling in regulating epithelial plasticity via crosstalk with the JNK pathway. |
format | Online Article Text |
id | pubmed-5495517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54955172017-07-18 Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure Kushnir, Tatyana Mezuman, Sharon Bar-Cohen, Shaked Lange, Rotem Paroush, Ze'ev Helman, Aharon PLoS Genet Research Article Dorsal closure (DC) is a developmental process in which two contralateral epithelial sheets migrate to seal a large hole in the dorsal ectoderm of the Drosophila embryo. Two signaling pathways act sequentially to orchestrate this dynamic morphogenetic process. First, c-Jun N-terminal kinase (JNK) signaling activity in the dorsal-most leading edge (LE) cells of the epidermis induces expression of decapentaplegic (dpp). Second, Dpp, a secreted TGF-β homolog, triggers cell shape changes in the adjacent, ventrally located lateral epidermis, that guide the morphogenetic movements and cell migration mandatory for DC. Here we uncover a cell non-autonomous requirement for the Epidermal growth factor receptor (Egfr) pathway in the lateral epidermis for sustained dpp expression in the LE. Specifically, we demonstrate that Egfr pathway activity in the lateral epidermis prevents expression of the gene scarface (scaf), encoding a secreted antagonist of JNK signaling. In embryos with compromised Egfr signaling, upregulated Scaf causes reduction of JNK activity in LE cells, thereby impeding completion of DC. Our results identify a new developmental role for Egfr signaling in regulating epithelial plasticity via crosstalk with the JNK pathway. Public Library of Science 2017-06-19 /pmc/articles/PMC5495517/ /pubmed/28628612 http://dx.doi.org/10.1371/journal.pgen.1006860 Text en © 2017 Kushnir et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kushnir, Tatyana Mezuman, Sharon Bar-Cohen, Shaked Lange, Rotem Paroush, Ze'ev Helman, Aharon Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure |
title | Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure |
title_full | Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure |
title_fullStr | Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure |
title_full_unstemmed | Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure |
title_short | Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure |
title_sort | novel interplay between jnk and egfr signaling in drosophila dorsal closure |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5495517/ https://www.ncbi.nlm.nih.gov/pubmed/28628612 http://dx.doi.org/10.1371/journal.pgen.1006860 |
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