Simple and highly efficient BAC recombineering using galK selection

Recombineering allows DNA cloned in Escherichia coli to be modified via lambda (λ) Red-mediated homologous recombination, obviating the need for restriction enzymes and DNA ligases to modify DNA. Here, we describe the construction of three new recombineering strains (SW102, SW105 and SW106) that all...

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Detalles Bibliográficos
Autores principales: Warming, Søren, Costantino, Nina, Court, Donald L., Jenkins, Nancy A., Copeland, Neal G.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549575/
https://www.ncbi.nlm.nih.gov/pubmed/15731329
http://dx.doi.org/10.1093/nar/gni035

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549575/
https://www.ncbi.nlm.nih.gov/pubmed/15731329
http://dx.doi.org/10.1093/nar/gni035

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