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Targeted ‘knockdown’ of spliceosome function in mammalian cells
The existence of two sophisticated parallel splicing machineries in multicellular organisms has raised intriguing questions—ranging from their impact on proteome expansion to the evolution of splicing and of metazoan genomes. Exploring roles for the distinct splicing systems in vivo has, however, be...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549580/ https://www.ncbi.nlm.nih.gov/pubmed/15731334 http://dx.doi.org/10.1093/nar/gni041 |
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author | Matter, Nathalie König, Harald |
author_facet | Matter, Nathalie König, Harald |
author_sort | Matter, Nathalie |
collection | PubMed |
description | The existence of two sophisticated parallel splicing machineries in multicellular organisms has raised intriguing questions—ranging from their impact on proteome expansion to the evolution of splicing and of metazoan genomes. Exploring roles for the distinct splicing systems in vivo has, however, been restricted by the lack of techniques to selectively inhibit their function in cells. In this study, we show that morpholino oligomers complementary to the branch-site recognition elements of U2 or U12 small nuclear RNA specifically suppress the function of the two splicing systems in mammalian cells. The data provide the first evidence for a role of distinct spliceosomes in pre-mRNA splicing from endogenous mammalian genes and establish a tool to define roles for the different splicing machineries in vivo. |
format | Text |
id | pubmed-549580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-5495802005-02-26 Targeted ‘knockdown’ of spliceosome function in mammalian cells Matter, Nathalie König, Harald Nucleic Acids Res Methods Online The existence of two sophisticated parallel splicing machineries in multicellular organisms has raised intriguing questions—ranging from their impact on proteome expansion to the evolution of splicing and of metazoan genomes. Exploring roles for the distinct splicing systems in vivo has, however, been restricted by the lack of techniques to selectively inhibit their function in cells. In this study, we show that morpholino oligomers complementary to the branch-site recognition elements of U2 or U12 small nuclear RNA specifically suppress the function of the two splicing systems in mammalian cells. The data provide the first evidence for a role of distinct spliceosomes in pre-mRNA splicing from endogenous mammalian genes and establish a tool to define roles for the different splicing machineries in vivo. Oxford University Press 2005 2005-02-24 /pmc/articles/PMC549580/ /pubmed/15731334 http://dx.doi.org/10.1093/nar/gni041 Text en © The Author 2005. Published by Oxford University Press. All rights reserved |
spellingShingle | Methods Online Matter, Nathalie König, Harald Targeted ‘knockdown’ of spliceosome function in mammalian cells |
title | Targeted ‘knockdown’ of spliceosome function in mammalian cells |
title_full | Targeted ‘knockdown’ of spliceosome function in mammalian cells |
title_fullStr | Targeted ‘knockdown’ of spliceosome function in mammalian cells |
title_full_unstemmed | Targeted ‘knockdown’ of spliceosome function in mammalian cells |
title_short | Targeted ‘knockdown’ of spliceosome function in mammalian cells |
title_sort | targeted ‘knockdown’ of spliceosome function in mammalian cells |
topic | Methods Online |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549580/ https://www.ncbi.nlm.nih.gov/pubmed/15731334 http://dx.doi.org/10.1093/nar/gni041 |
work_keys_str_mv | AT matternathalie targetedknockdownofspliceosomefunctioninmammaliancells AT konigharald targetedknockdownofspliceosomefunctioninmammaliancells |