Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli

BACKGROUND: Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the...

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Detalles Bibliográficos
Autores principales: Jaén, Karim E., Sigala, Juan-Carlos, Olivares-Hernández, Roberto, Niehaus, Karsten, Lara, Alvaro R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496438/
https://www.ncbi.nlm.nih.gov/pubmed/28676110
http://dx.doi.org/10.1186/s12896-017-0378-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496438/
https://www.ncbi.nlm.nih.gov/pubmed/28676110
http://dx.doi.org/10.1186/s12896-017-0378-x

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