Protein-bound polyphenols create “ghost” band artifacts during chemiluminescence-based antigen detection

Antigen detection during Western blotting commonly utilizes a horseradish peroxidase-coupled secondary antibody and enhanced chemiluminescent substrate. We utilized this technique to examine the impact of green tea-derived polyphenols on the binding of egg white protein-specific IgE antibodies from...

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Detalles Bibliográficos
Autores principales: Plundrich, Nathalie, Lila, Mary Ann, Foegeding, Edward, Laster, Scott
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2017
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497812/
https://www.ncbi.nlm.nih.gov/pubmed/28721200
http://dx.doi.org/10.12688/f1000research.10622.2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497812/
https://www.ncbi.nlm.nih.gov/pubmed/28721200
http://dx.doi.org/10.12688/f1000research.10622.2

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