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Protein-bound polyphenols create “ghost” band artifacts during chemiluminescence-based antigen detection
Antigen detection during Western blotting commonly utilizes a horseradish peroxidase-coupled secondary antibody and enhanced chemiluminescent substrate. We utilized this technique to examine the impact of green tea-derived polyphenols on the binding of egg white protein-specific IgE antibodies from...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
F1000Research
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497812/ https://www.ncbi.nlm.nih.gov/pubmed/28721200 http://dx.doi.org/10.12688/f1000research.10622.2 |