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Quantitative characterization of the auxin-inducible degron: a guide for dynamic protein depletion in single yeast cells

Perturbations are essential for the interrogation of biological systems. The auxin-inducible degron harbors great potential for dynamic protein depletion in yeast. Here, we thoroughly and quantitatively characterize the auxin-inducible degron in single yeast cells. We show that an auxin concentratio...

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Detalles Bibliográficos
Autores principales: Papagiannakis, Alexandros, de Jonge, Janeska J, Zhang, Zheng, Heinemann, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498663/
https://www.ncbi.nlm.nih.gov/pubmed/28680098
http://dx.doi.org/10.1038/s41598-017-04791-6