Whole Genome Amplification of Day 3 or Day 5 Human Embryos Biopsies Provides a Suitable DNA Template for PCR-Based Techniques for Genotyping, a Complement of Preimplantation Genetic Testing

Our objective was to determine if whole genome amplification (WGA) provides suitable DNA for qPCR-based genotyping for human embryos. Single blastomeres (Day 3) or trophoblastic cells (Day 5) were isolated from 342 embryos for WGA. Comparative Genomic Hybridization determined embryo sex as well as T...

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Detalles Bibliográficos
Autores principales: Schaeffer, Elizabeth, López-Bayghen, Bruno, Neumann, Adina, Porchia, Leonardo M., Camacho, Rafael, Garrido, Efraín, Gómez, Rocío, Camargo, Felipe, López-Bayghen, Esther
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498896/
https://www.ncbi.nlm.nih.gov/pubmed/28717645
http://dx.doi.org/10.1155/2017/1209158

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498896/
https://www.ncbi.nlm.nih.gov/pubmed/28717645
http://dx.doi.org/10.1155/2017/1209158

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