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Development of a counterselectable seamless mutagenesis system in lactic acid bacteria

BACKGROUND: Lactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector-based double-crossover strategies, which are laborious and inefficient. To add...

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Detalles Bibliográficos
Autores principales: Xin, Yongping, Guo, Tingting, Mu, Yingli, Kong, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499019/
https://www.ncbi.nlm.nih.gov/pubmed/28679374
http://dx.doi.org/10.1186/s12934-017-0731-8