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Development of a counterselectable seamless mutagenesis system in lactic acid bacteria
BACKGROUND: Lactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector-based double-crossover strategies, which are laborious and inefficient. To add...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499019/ https://www.ncbi.nlm.nih.gov/pubmed/28679374 http://dx.doi.org/10.1186/s12934-017-0731-8 |