Chemical ligation of oligonucleotides using an electrophilic phosphorothioester

We developed a new approach for chemical ligation of oligonucleotides using the electrophilic phosphorothioester (EPT) group. A nucleophilic phosphorothioate group on oligonucleotides was converted into the EPT group by treatment with Sanger's reagent (1-fluoro-2,4-dinitrobenzene). EPT oligonuc...

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Detalles Bibliográficos
Autores principales: Maruyama, Hideto, Oikawa, Ryota, Hayakawa, Mayu, Takamori, Shono, Kimura, Yasuaki, Abe, Naoko, Tsuji, Genichiro, Matsuda, Akira, Shuto, Satoshi, Ito, Yoshihiro, Abe, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499596/
https://www.ncbi.nlm.nih.gov/pubmed/28520986
http://dx.doi.org/10.1093/nar/gkx459

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499596/
https://www.ncbi.nlm.nih.gov/pubmed/28520986
http://dx.doi.org/10.1093/nar/gkx459

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