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Chemical ligation of oligonucleotides using an electrophilic phosphorothioester

We developed a new approach for chemical ligation of oligonucleotides using the electrophilic phosphorothioester (EPT) group. A nucleophilic phosphorothioate group on oligonucleotides was converted into the EPT group by treatment with Sanger's reagent (1-fluoro-2,4-dinitrobenzene). EPT oligonuc...

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Detalles Bibliográficos
Autores principales:	Maruyama, Hideto, Oikawa, Ryota, Hayakawa, Mayu, Takamori, Shono, Kimura, Yasuaki, Abe, Naoko, Tsuji, Genichiro, Matsuda, Akira, Shuto, Satoshi, Ito, Yoshihiro, Abe, Hiroshi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499596/ https://www.ncbi.nlm.nih.gov/pubmed/28520986 http://dx.doi.org/10.1093/nar/gkx459

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