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A novel rare sugar inhibitor of murine herpes simplex keratitis

PURPOSE: To determine the therapeutic efficacy of a novel rare sugar, L-psicose, for the treatment of HSV-1 induced herpetic stromal keratitis (HSK) in a mouse eye model. METHODS: One rare sugar L-psicose was assayed for HSV-1 inhibition of in vitro virus adsorption. The IC(50) and IC(90) values of...

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Autores principales: Muniruzzaman, Syed, McIntosh, Megan, Hossain, Ahamed, Izumori, Ken, Bhattacharjee, Partha S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499707/
https://www.ncbi.nlm.nih.gov/pubmed/27262904
http://dx.doi.org/10.1016/j.jphs.2016.05.004
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author Muniruzzaman, Syed
McIntosh, Megan
Hossain, Ahamed
Izumori, Ken
Bhattacharjee, Partha S.
author_facet Muniruzzaman, Syed
McIntosh, Megan
Hossain, Ahamed
Izumori, Ken
Bhattacharjee, Partha S.
author_sort Muniruzzaman, Syed
collection PubMed
description PURPOSE: To determine the therapeutic efficacy of a novel rare sugar, L-psicose, for the treatment of HSV-1 induced herpetic stromal keratitis (HSK) in a mouse eye model. METHODS: One rare sugar L-psicose was assayed for HSV-1 inhibition of in vitro virus adsorption. The IC(50) and IC(90) values of L-psicose were determined using plaque reduction assay (PRA) in CV-1 cell. Female Balb/c mice were corneally infected with HSV-1, strain KOS-GFP; A topical eye drop treatment of L-psicose was started 24 h after infection and continued four times daily for ten consecutive days. The severity of HSK was monitored by slit lamp examination in a masked fashion and Infectious HSV-1 shedding was determined by PRA. RESULTS: L-psicose was found to have anti-viral activity in vitro at an IC(50) dose of 99.5 mM and an IC(90) dose of 160 mM. Topical eye drop treatment with 200 mM L-psicose in PBS solution significantly reduced the severity of HSK compared to the mock treatment group. The in vivo mouse ocular model results of L-psicose therapy correlated with accelerated clearance of virus from eye swabs. CONCLUSION: The results suggest that topical treatment with rare sugar L-psicose has efficacy against HSK through inhibition of HSV-1.
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spelling pubmed-54997072017-07-06 A novel rare sugar inhibitor of murine herpes simplex keratitis Muniruzzaman, Syed McIntosh, Megan Hossain, Ahamed Izumori, Ken Bhattacharjee, Partha S. J Pharmacol Sci Article PURPOSE: To determine the therapeutic efficacy of a novel rare sugar, L-psicose, for the treatment of HSV-1 induced herpetic stromal keratitis (HSK) in a mouse eye model. METHODS: One rare sugar L-psicose was assayed for HSV-1 inhibition of in vitro virus adsorption. The IC(50) and IC(90) values of L-psicose were determined using plaque reduction assay (PRA) in CV-1 cell. Female Balb/c mice were corneally infected with HSV-1, strain KOS-GFP; A topical eye drop treatment of L-psicose was started 24 h after infection and continued four times daily for ten consecutive days. The severity of HSK was monitored by slit lamp examination in a masked fashion and Infectious HSV-1 shedding was determined by PRA. RESULTS: L-psicose was found to have anti-viral activity in vitro at an IC(50) dose of 99.5 mM and an IC(90) dose of 160 mM. Topical eye drop treatment with 200 mM L-psicose in PBS solution significantly reduced the severity of HSK compared to the mock treatment group. The in vivo mouse ocular model results of L-psicose therapy correlated with accelerated clearance of virus from eye swabs. CONCLUSION: The results suggest that topical treatment with rare sugar L-psicose has efficacy against HSK through inhibition of HSV-1. 2016-05-20 2016-06 /pmc/articles/PMC5499707/ /pubmed/27262904 http://dx.doi.org/10.1016/j.jphs.2016.05.004 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Muniruzzaman, Syed
McIntosh, Megan
Hossain, Ahamed
Izumori, Ken
Bhattacharjee, Partha S.
A novel rare sugar inhibitor of murine herpes simplex keratitis
title A novel rare sugar inhibitor of murine herpes simplex keratitis
title_full A novel rare sugar inhibitor of murine herpes simplex keratitis
title_fullStr A novel rare sugar inhibitor of murine herpes simplex keratitis
title_full_unstemmed A novel rare sugar inhibitor of murine herpes simplex keratitis
title_short A novel rare sugar inhibitor of murine herpes simplex keratitis
title_sort novel rare sugar inhibitor of murine herpes simplex keratitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499707/
https://www.ncbi.nlm.nih.gov/pubmed/27262904
http://dx.doi.org/10.1016/j.jphs.2016.05.004
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