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Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease

Nonsense-mediated mRNA decay (NMD) is essential for removing premature termination codon-containing transcripts from cells. Studying the NMD pathway in model organisms can help to elucidate the NMD mechanism in humans and improve our understanding of how this biologically important process has evolv...

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Autores principales: Tian, Miao, Yang, Wentao, Zhang, Jing, Dang, Huai, Lu, Xingyi, Fu, Chengjie, Miao, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499736/
https://www.ncbi.nlm.nih.gov/pubmed/28402567
http://dx.doi.org/10.1093/nar/gkx256
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author Tian, Miao
Yang, Wentao
Zhang, Jing
Dang, Huai
Lu, Xingyi
Fu, Chengjie
Miao, Wei
author_facet Tian, Miao
Yang, Wentao
Zhang, Jing
Dang, Huai
Lu, Xingyi
Fu, Chengjie
Miao, Wei
author_sort Tian, Miao
collection PubMed
description Nonsense-mediated mRNA decay (NMD) is essential for removing premature termination codon-containing transcripts from cells. Studying the NMD pathway in model organisms can help to elucidate the NMD mechanism in humans and improve our understanding of how this biologically important process has evolved. Ciliates are among the earliest branching eukaryotes; their NMD mechanism is poorly understood and may be primordial. We demonstrate that highly conserved Upf proteins (Upf1a, Upf2 and Upf3) are involved in the NMD pathway of the ciliate, Tetrahymena thermophila. We further show that a novel protozoa-specific nuclease, Smg6L, is responsible for destroying many NMD-targeted transcripts. Transcriptome-wide identification and characterization of NMD-targeted transcripts in vegetative Tetrahymena cells showed that many have exon–exon junctions downstream of the termination codon. However, Tetrahymena may lack a functional exon junction complex (EJC), and the Tetrahymena ortholog of an EJC core component, Mago nashi (Mag1), is dispensable for NMD. Therefore, NMD is EJC independent in this early branching eukaryote.
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spelling pubmed-54997362017-07-10 Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease Tian, Miao Yang, Wentao Zhang, Jing Dang, Huai Lu, Xingyi Fu, Chengjie Miao, Wei Nucleic Acids Res RNA Nonsense-mediated mRNA decay (NMD) is essential for removing premature termination codon-containing transcripts from cells. Studying the NMD pathway in model organisms can help to elucidate the NMD mechanism in humans and improve our understanding of how this biologically important process has evolved. Ciliates are among the earliest branching eukaryotes; their NMD mechanism is poorly understood and may be primordial. We demonstrate that highly conserved Upf proteins (Upf1a, Upf2 and Upf3) are involved in the NMD pathway of the ciliate, Tetrahymena thermophila. We further show that a novel protozoa-specific nuclease, Smg6L, is responsible for destroying many NMD-targeted transcripts. Transcriptome-wide identification and characterization of NMD-targeted transcripts in vegetative Tetrahymena cells showed that many have exon–exon junctions downstream of the termination codon. However, Tetrahymena may lack a functional exon junction complex (EJC), and the Tetrahymena ortholog of an EJC core component, Mago nashi (Mag1), is dispensable for NMD. Therefore, NMD is EJC independent in this early branching eukaryote. Oxford University Press 2017-06-20 2017-04-11 /pmc/articles/PMC5499736/ /pubmed/28402567 http://dx.doi.org/10.1093/nar/gkx256 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle RNA
Tian, Miao
Yang, Wentao
Zhang, Jing
Dang, Huai
Lu, Xingyi
Fu, Chengjie
Miao, Wei
Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease
title Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease
title_full Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease
title_fullStr Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease
title_full_unstemmed Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease
title_short Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease
title_sort nonsense-mediated mrna decay in tetrahymena is ejc independent and requires a protozoa-specific nuclease
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499736/
https://www.ncbi.nlm.nih.gov/pubmed/28402567
http://dx.doi.org/10.1093/nar/gkx256
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