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Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method

DNA assembly forms the cornerstone of modern synthetic biology. Despite the numerous available methods, scarless multi-fragment assembly of large plasmids remains challenging. Furthermore, the upcoming wave in molecular biological automation demands a rethinking of how we perform DNA assembly. To st...

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Detalles Bibliográficos
Autores principales: Liang, Jing, Liu, Zihe, Low, Xi Z., Ang, Ee L., Zhao, Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499748/
https://www.ncbi.nlm.nih.gov/pubmed/28334760
http://dx.doi.org/10.1093/nar/gkx132