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Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method
DNA assembly forms the cornerstone of modern synthetic biology. Despite the numerous available methods, scarless multi-fragment assembly of large plasmids remains challenging. Furthermore, the upcoming wave in molecular biological automation demands a rethinking of how we perform DNA assembly. To st...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499748/ https://www.ncbi.nlm.nih.gov/pubmed/28334760 http://dx.doi.org/10.1093/nar/gkx132 |