DNA isolation protocol effects on nuclear DNA analysis by microarrays, droplet digital PCR, and whole genome sequencing, and on mitochondrial DNA copy number estimation

Potential bias introduced during DNA isolation is inadequately explored, although it could have significant impact on downstream analysis. To investigate this in human brain, we isolated DNA from cerebellum and frontal cortex using spin columns under different conditions, and salting-out. We first a...

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Detalles Bibliográficos
Autores principales: Nacheva, Elizabeth, Mokretar, Katya, Soenmez, Aynur, Pittman, Alan M., Grace, Colin, Valli, Roberto, Ejaz, Ayesha, Vattathil, Selina, Maserati, Emanuela, Houlden, Henry, Taanman, Jan-Willem, Schapira, Anthony H., Proukakis, Christos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500342/
https://www.ncbi.nlm.nih.gov/pubmed/28683077
http://dx.doi.org/10.1371/journal.pone.0180467

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500342/
https://www.ncbi.nlm.nih.gov/pubmed/28683077
http://dx.doi.org/10.1371/journal.pone.0180467

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