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WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins

Post-translational modification with O-linked β-N-acetylglucosamine (O-GlcNAc) occurs selectively on serine and/or threonine residues of cytoplasmic and nuclear proteins, and dynamically regulates their molecular functions. Since conventional strategies to evaluate the O-GlcNAcylation level of a spe...

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Detalles Bibliográficos
Autores principales: Kubota, Yuji, Fujioka, Ko, Takekawa, Mutsuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501588/
https://www.ncbi.nlm.nih.gov/pubmed/28686627
http://dx.doi.org/10.1371/journal.pone.0180714