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WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins
Post-translational modification with O-linked β-N-acetylglucosamine (O-GlcNAc) occurs selectively on serine and/or threonine residues of cytoplasmic and nuclear proteins, and dynamically regulates their molecular functions. Since conventional strategies to evaluate the O-GlcNAcylation level of a spe...
Autores principales: | Kubota, Yuji, Fujioka, Ko, Takekawa, Mutsuhiro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501588/ https://www.ncbi.nlm.nih.gov/pubmed/28686627 http://dx.doi.org/10.1371/journal.pone.0180714 |
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