Biochemical properties of GH94 cellodextrin phosphorylase THA_1941 from a thermophilic eubacterium Thermosipho africanus TCF52B with cellobiose phosphorylase activity

A hypothetic gene (THA_1941) encoding a putative cellobiose phosphorylase (CBP) from Thermosipho africanus TCF52B has very low amino acid identities (less than 12%) to all known GH94 enzymes. This gene was cloned and over-expressed in Escherichia coli BL21(DE3). The recombinant protein was hypothesi...

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Detalles Bibliográficos
Autores principales: Wu, Yuanyuan, Mao, Guotao, Fan, Haiyan, Song, Andong, Zhang, Yi-Heng Percival, Chen, Hongge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501786/
https://www.ncbi.nlm.nih.gov/pubmed/28687766
http://dx.doi.org/10.1038/s41598-017-05289-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501786/
https://www.ncbi.nlm.nih.gov/pubmed/28687766
http://dx.doi.org/10.1038/s41598-017-05289-x

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